On January 24, 2023, Mark Stoeckle presented our recent work on measuring marine fish eDNA abundance at the sixth annual Environmental DNA Technical Exchange Workshop (6eDTEW), sponsored by interagency US Government eDNA Working Group.
For its 2021 Annual Review, the Andrew W. Marshall Foundation presented an hour-long seminar in which Jesse Ausubel speaks briefly on experimentation, Melissa Flagg on intellectual courage, and Dan Patt on mentorship, followed by Q&A. IGood session. Jesse’s 5 minutes of remarks, titled Don’t Do Anything for the First Time, begin 15 minutes into the session following Jaymie Durnan’s opening review.
Thanks to Tracy Gill and Katharine Egan, Mark Stoeckle and Jesse Ausubel gave a NOAA-wide “Omics” seminar. 29 October 2021. Title and abstract are below. View the recording of the webinar via Adobe Connect, here: https://noaabroadcast.adobeconnect.com/pnnos0mcsh3z/ Thanks to the attendees for lotss of great questions and the lively to-and-fro in the Chat Box.
Abstract: Measuring quantities of eDNA is fast becoming a preferred method of learning the presence and abundance of fish and other aquatic species. But how much water need one filter and how much DNA need one process to obtain a reasonably complete and reproducible answer? Tests of an eDNA metabarcoding protocol for marine bony fish show more water, more species of fish up to levels tested. Amplifying decreasing amounts of extracted DNA yields progressively fewer species. Species represented by more copies (reads) of their DNA are detected more reproducibly and with less variation than lower-read species. Findings are consistent with Poisson distribution of rarer eDNA. We also vary PCR cycles, sequencing depth, primer concentrations, and primers. Our findings have multiple practical implications, including for survey strategies for both common and rare species, and identify some limits of knowledge and research directions for aquatic eDNA science.
The Ocean session starts at 1:57 and lasts to 3:09. Jesse’s opening remarks (about better measurement of abundance of ocean life) start at 2:07 (3 minutes) and his pitch for soundscapes is at 2:41:35 (1.5 minutes). The entire session is good listening (and some video too).
The Consortium for Ocean Leadership (COL) hosted a virtual symposium entitled Observing Life in a Changing Ocean: Exploring a ‘Census of Marine Life’ Today, on January 27, 2021.
The Census of Marine Life was an international program of discovery of life in the ocean, from microbes to whales and from coral reefs to abyssal plains. The Census ran from 2000-2010 and was a model for building collaboration and a global baseline of knowledge of marine diversity, distribution, and abundance. COL convened the symposium to highlight the need and generate excitement for a sustained, collaborative, and systematic program in marine biodiversity research and observation. Jesse Ausubel gave an opening 25-minute retrospective on the program beginning 5 minutes 40 seconds into the video.