Mark Stoeckle and Jesse Ausubel presented in the NOAA ‘Omics Seminar Series on Marine fish eDNA Metabarcoding: Promising Developments and Early Applications. The outline:
–eDNA abundance matters (relevant to detection, quantification, field design, laboratory protocols)
–Adding internal standard to metabarcoding PCRs quantifies eDNA (converts relative sequence reads to absolute eDNA copies)
–Current marine fish metabarcoding protocols ready for wider use (reasonably accurate index of fish abundance, especially for more abundant species)
–eDNA metabarcoding can overcome information hurdles for ecosystem-based management
A recording is here , 40-minute presentation and 20-minute Q&A. Thanks to NOAA’s Katharine Egan and Nicole Miller.