News coverage about surprising Jersey Shore fish spotted w eDNA

National Fisherman May 14, 2020 Genetic markers reveal Brazilian cownose rays, Gulf kingfish in New Jersey waters Kirk Moore

Agencia EFE (via El Diario, Spain) El analísis de ADN medioambiental detecta migraciones de especies marinas https://www.eldiario.es/sociedad/analisis-ADN-medioambiental-migraciones-especies_0_1026448521.html

Prensa Latina, Cuba Descubren nuevos patrones de migración de especies marinas tropicales https://www.prensa-latina.cu/index.php?o=rn&id=365583&SEO=descubren-nuevos-patrones-de-migracion-de-especies-marinas-tropicales

Globedia, Spain Detectan migraciones de especies marinas gracias al ADN medioambiental http://globedia.com/detectan-migraciones-especies-marinas-gracias-adn-medioambiental

Revista Planeta, Brazil  Arraia típica do Brasil está chegando perto da costa de Nova York https://www.revistaplaneta.com.br/arraia-tipica-do-brasil-esta-chegando-perto-da-costa-de-nova-york/

DIVE Magazine, UK DNA Traces Prove To Be Useful Tool in Understanding Fish Populations http://divemagazine.co.uk/eco/8973-tracking-and-tracing-in-the-ocean-using-dna-residue

New PHE paper about surprising species found off New Jersey

Front. Mar. Sci., 05 May 2020 | https://doi.org/10.3389/fmars.2020.00226

Improved Environmental DNA Reference Library Detects Overlooked Marine Fishes in New Jersey, United States

Mark Y. Stoeckle*, Mithun Das Mishu and Zachary Charlop-Powers

  • Program for the Human Environment, The Rockefeller University, New York, NY, United States

An accurate, comprehensive reference sequence library maximizes information gained from environmental DNA (eDNA) metabarcoding of marine fishes. Here, we used a regional checklist and early results from an ongoing eDNA time series to target mid-Atlantic U.S. coastal fishes lacking reference sequences. We obtained 60 specimens representing 31 species from NOAA trawl surveys and institutional collections, and analyzed 12S and COI barcode regions, the latter to confirm specimen identification. Combined with existing GenBank accessions, the enhanced 12S dataset covered most (74%) of 341 fishes on New Jersey State checklist including 95% of those categorized abundant or common. For eDNA time series, we collected water samples approximately twice monthly for 24 months at an ocean and a bay site in New Jersey. Metabarcoding was performed using separate 12S primer sets targeting bony and cartilaginous fishes. Bioinformatic analysis of Illumina MiSeq fastq files with the augmented library yielded exact matches for 90% of the 104 fish amplicon sequence variants generated from field samples. Newly obtained reference sequences revealed two southern U.S. species as relatively common warm season migrants: Gulf kingfish (Menticirrhus littoralis) and Brazilian cownose ray (Rhinoptera brasiliensis). A beach wrack specimen corroborated the local presence of Brazilian cownose ray. Our results highlight the value of strengthening reference libraries and demonstrate that eDNA can help detect range shifts including those of species overlooked by traditional surveys.

Whales and eDNA in NY waters Podcast

NYU science journalism grad student Kaitlyn Jeanne Nichols’s podcast follows the return of whales to NY and interviews Mark on how eDNA is helping reveal animal life in NYC waters. https://soundcloud.com/user-833449477/chasing-whales-in-new-york-city (segment starts at 7:30).