We post a Schematic of an eDNA metabarcoding protocol for marine fish.
We recently showed that current laboratory protocols optimize sensitivity and reproducibility, especially for more abundant fish populations (ICES J Mar Sci 2022). eDNA metabarcoding is generally considered a qualitative tool for relative abundance. We hope to help develop eDNA metabarcoding as a quantitative tool for absolute abundance (link to pdf work-in-progress).