The Barcode Blog

A mostly scientific blog about short DNA sequences for species identification and discovery. I encourage your commentary. -- Mark Stoeckle

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Establishing a DNA standard barcode for land plants

Kew Scientist April 2005Plant researchers from 11 world herbaria are investigating DNA regions for their potential as barcodes for land plant species. From the project rationale: “although the mitochondrial gene region, CO1 ( cox1 ), has already been used with considerable success across a range of animal groups and shows promise in at least some algal groups, it is characterized by relatively low rates of sequence divergence in land plants. Mitochondrial DNA in land plants also undergoes rearrangements, exhibits incorporation of foreign genes and frequent transfer of some genes to the nuclear genome. It is therefore desirable to find an alternative region or, if necessary, regions from one of the other genomes that would be suitable as a barcode.”

This project aims to establish a standard DNA barcode for land plants. Phase 1, completed in December 2005, was a survey of regions that have potential as land plant barcodes. Phase 2, to be completed by January 2007, is to “ground test” the most promising regions in a series of parallel case studies that incoporates representatives of all major land plant lineages.

If this competition is successful, it should be relatively straightforward (ie fast and inexpensive) to compile a comprehensive library of plant DNA barcodes, as there are only about 500,000 known plant species, the world catalog is thought to be essentially complete, and there are several herbaria with large specimen and DNA collections.

This entry was posted on Wednesday, July 26th, 2006 at 9:07 pm and is filed under General. You can follow any responses to this entry through the RSS 2.0 feed. Both comments and pings are currently closed.

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Contact: mark.stoeckle@rockefeller.edu

About this site

This web site is an outgrowth of the Taxonomy, DNA, and Barcode of Life meeting held at Banbury Center, Cold Spring Harbor Laboratory, September 9-12, 2003. It is designed and managed by Mark Stoeckle, Perrin Meyer, and Jason Yung at the Program for the Human Environment (PHE) at The Rockefeller University.

About the Program for the Human Environment

The involvement of the Program for the Human Environment in DNA barcoding dates to Jesse Ausubel's attendance in February 2002 at a conference in Nova Scotia organized by the Canadian Center for Marine Biodiversity. At the conference, Paul Hebert presented for the first time his concept of large-scale DNA barcoding for species identification. Impressed by the potential for this technology to address difficult challenges in the Census of Marine Life, Jesse agreed with Paul on encouraging a conference to explore the contribution taxonomy and DNA could make to the Census as well as other large-scale terrestrial efforts. In his capacity as a Program Director of the Sloan Foundation, Jesse turned to the Banbury Conference Center of Cold Spring Harbor Laboratory, whose leader Jan Witkowski prepared a strong proposal to explore both the scientific reliability of barcoding and the processes that might bring it to broad application. Concurrently, PHE researcher Mark Stoeckle began to work with the Hebert lab on analytic studies of barcoding in birds. Our involvement in barcoding now takes 3 forms: assisting the organizational development of the Consortium for the Barcode of Life and the Barcode of Life Initiative; contributing to the scientific development of the field, especially by studies in birds, and contributing to public understanding of the science and technology of barcoding and its applications through improved visualization techniques and preparation of brochures and other broadly accessible means, including this website. While the Sloan Foundation continues to support CBOL through a grant to the Smithsonian Institution, it does not provide financial support for barcoding research itself or support to the PHE for its research in this field.