The Barcode Blog

Knowledge of how species are distributed is essential for understanding evolution and ecology, and monitoring enables detecting invasive species and recognizing effects of biological and physical environmental change. That’s easy to say, but many species are small, secretive, or difficult to distinguish from one another, so mapping species distributions requires enormous human effort and ongoing monitoring requires even more. I venture a guess that we have good monitoring for 10,000 or so plant and animal species, mostly large animals and those plants and animals of economic importance, and static distribution maps for about 100,000 species, out of a total of 1.7 million named species and not counting the projected total of 10 million species that might eventually be recognized when surveying biodiversity approaches completion. 

Just as high-resolution satellite mapping has surpassed most ground surveys in accuracy, speed, and cost, we need efficient technologies that can help detect and monitor species from environmental samples. In 9 April 2008 Early Online Biol Lett researchers from Universite Joseph Fourier and Universite de Savoie, France, and Universita Milano Bicocca, Italy, apply high-sensitivity DNA analysis to detect presence or absence of American Bullfrog Rana catesbeiana, a globally invasive species. PCR amplification of a diagnostic 79 bp fragment of mitochondrial gene cyt b using species-specific primers (no amplification of samples from the 5 locally native Rana sp). Three 15 mL water samples were collected from each of 9 ponds (surface area 1000-10,000 m^2) in France, including “three ponds where bullfrogs were present at low density (one to two adults seen, no reproduction), three ponds where bullfrogs were present at high density (dozens of adults and thousands of tadpoles), and three ponds where bullfrogs have never been detected.” Each sample was analyzed 3-5 times, giving 9-15 repeats per pond. R. catesbiana DNA was never detected in the ponds with no bullfrogs and was detected in water samples from all three high-density ponds, with most (79%) of replications positive. Bullfrog DNA was also detected in all low-density ponds, although fewer of the replications were postive ( 37%).

Ficetola et al observe “our approach allows the reliable detection of secretive organisms in wetlands without direct observation.”  The authors conclude “The ongoing effort to develop DNA barcodes for identifying species  from degraded DNA (Hajibabaei et al 2006; Taberlet et al 2007) will make our approach applicable to more and more plant and animals species…These factors will soon make possible the assessment of the current biodiversity of macro-organisms from environmental samples.” 

Like satellite mapping 20 years ago, DNA-based environmental monitoring of biodiversity, aided by growing DNA barcode libraries, is set to expand rapidly.

This entry was posted on Friday, April 25th, 2008 at 4:29 am and is filed under General. You can follow any responses to this entry through the RSS 2.0 feed. Both comments and pings are currently closed.